Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by γ-irradiation (60Co) of adventitious roots – Accepted Manuscript

Abstract: We established an efficient in vitro protocol for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calli on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-D. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid (GA3) and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L GA3. The shoots developed into plants with well-developed taproots on 1/3 strength Schenk and Hildebrandt (SH) basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid (NAA). When the plants transferred to soil, about 30% of the regenerated plants developed into normal plants.