20(S)-protopanaxadiol 20-O-D-glucopyranoside, or compound K (CK), exerts antidiabetic effects that are mediated by insulin secretion through KATP channels in pancreatic β-cells. However, the antidiabetic effects of CK may be limited due to its low bioavailability. Methods: In this study, we aimed to enhance the antidiabetic activity and lower the toxicity of CK by conjugation with β-cyclodextrin (CD) (CD-CK), and determined if the CD-CK compound enhanced pancreatic islet recovery compared with CK alone in an alloxan-induced zebrafish model.