Prenyltransferases catalyze the sequential addition of IPP units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cis-prenyltransferases (CPTs). The functions of CPTs have been relatively well characterized in bacteria, yeast, and mammals compared to that of in plants. The characterization of CPTs also has been less studied than TPTs. In this present study, molecular cloning and functional characterization of a CPT from a medicinal plant, Panax ginseng Mayer are addressed.