Saponins from Panax japonicus (SPJ) are the most abundant and main active components of Panax japonicus, which replaces Ginseng roots in treatment for many kinds of diseases in minority ethnic group in China. Our previous studies have demonstrated that SPJ has the effects of anti-inflammation through MAPK and NF-κB signaling pathway. The present study was designed to investigate whether SPJ can modulate intestine TJ barrier in aging rats and further to explore the potential mechanism.